Techniques for the standard histological and ultrastructural assessment of nerve biopsies


Joachim Weis, Istvan Katona, Stefan Nikolin, Lucilla Nobbio, Valeria Prada, Marina Grandis, Angelo Schenone

It is always a challenge to acquire a clear picture of the pathological processes and changes in any disease. For this purpose, it is advantageous to directly examine the affected organ. Nerve biopsy has been a method of choice for decades to classify peripheral neuropathies and to find clues to uncover their etiology. The histologic examination of the peripheral nerve provides information on axonal or myelin pathology as well as on the surrounding connective tissue and vascularization of the nerve. Minimal requirements of the workup include paraffin histology as well as resin semithin section histology. Cryostat sections, teased fiber preparations and electron microscopy are potentially useful in a subset of cases. Here we describe our standard procedures for the workup of the tissue sample and provide examples of diagnostically relevant findings.

Published: 26 November 2021
Journal: J Peripher Nerv Syst, volume 26, pages S3 - S10


This publication is recommended by the Neuromuscular Pathology Specialised Group group.

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